Application of Epic® System for Label-free High Throughput Screening and Cell-dependent Signaling Profiles of GPCR Ligands in Recombinant and Native Cells

Presenters:
Evi Kostenis, University of Bonn
Anthony Frutos, Corning Incorporated
GPCRs represent an important class of molecular targets for development of therapeutic agents. Emerging evidence suggests, however, that compound efficacy does not only depend on the ligand/receptor couple but also on the nature of available signaling pathways engaged by the individual receptor in each cell line. This talk will give selected examples showing effector-dependent efficacy of molecules affecting GPCR-behavior and address the potential usefulness of label free technologies in analyzing receptor signaling in recombinant and native cells.

Hit Validation Using Biolayer Interferometry

Presenter:
Frank Podlaski, Sr. Principal Scientist Hoffman LaRoche, Nutley, NJ.

A Fully Automated Workstation for High Throughput, High Sensitivity Surface Plasmon Resonance

Presenters:
Nobuhiko Ogura FUJIFILM Corporation
and Al Kolb 2KeyTech Solutions
Surface Plasmon Resonance (SPR) is an excellent method for the detailed study of biological binding reactions without the use of labels. However, the throughput has been too low to meet the demand of downstream processes in drug discovery such lead characterization and optimization. The introduction of the FujiFilm AP-3000 makes SPR practical for these applications. The AP-3000 is a fully automated workstation with 384-well plate compatibility and throughputs of 3,840 data points per day. This tutorial will present the technology behind the AP-3000 and data from collaborators showing that the sensitivity and performance of SPR are not compromised.

Facilitating Screening and Lead Characterization with Biacore Label-Free Analysis Systems

Presenter:
Markku Hamalainen, GE Healthcare, Sweden
(Dr Markku Hamalainen is senior scientist in chemometrics at GE Healthcare, Uppsala, Sweden) Biacore™ A100 supports drug discovery from screening to detailed characterization of leads. This tutorial will demonstrate the power and ease-of-use of Biacore systems for the efficient generation and analysis of compound-target binding data. The advantages of the method will be demonstrated, including low target consumption and ready provision of selectivity information.

The Cellkey™ Product Family: a Platform for The Full Range of Early Drug Discovery Applications

Presenter:
Ryan McGuinness. Senior Application Scientist. MDS Analytical Technologies
In this workshop, we highlight the CellKey™ System family of label-free cell based assay products. A detailed overview of this important technology platform is discussed as are its applications to the full spectrum of drug discovery, including target identification and validation, high throughput compound screening, and lead optimization.

The BIND Reader: A Universal Platform for Label-Free Drug Discovery

Presenter:
Lance Laing Ph.D. Director of Bioapplications
This presentation will introduce our new instrument in the BIND® platform and discuss advances in label-free cell-based and biochemical assays. The new BIND® Reader Turbo offers 1536-well capability, a significant increase in scan speed, and new software for integration with popular laboratory automation equipment. In kinetic mode, the Reader can monitor cellular responses across an entire plate every 10, 15, or 45 seconds for 96-, 384-, or 1536-well plates respectively, or in simple end point mode, can sample nearly a million wells in an 8 hour day. Designed from the ground up to be easily integrated into an automated workflow, the Reader uses biosensor plates in standard SBS formats. The open hardware architecture readily accepts plates fed by all popular robotic arms and interfaces with the most popular automation schedulers. Together, these enhancements enable monitoring cell responses on a short time scale using very low numbers of recombinant cell lines or primary cells. The flexibility of the system extends the applications to biochemical assay formats including fragment screening. The BIND technology provides a wide range of microplate formats and throughput to accommodate needs from assay development through to compound profiling and HTS. Data will be presented describing how the system can be applied to cell-based applications (endogenous GPCRs, primary cells, chemotaxis, and ion channels) and small molecule biochemical assays for fragment screening and lead profiling.